Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Applicable Secondary Antibody
SAA544Rb59, SAA544Rb58, SAA544Rb57, SAA544Rb18, SAA544Rb19
4℃ with ice bags
Polyclonal antibody preparation
CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
Activated Leukocyte Cell Adhesion Molecule
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Rabbit, Anti-Activated Leukocyte Adhesion Molecule (ALCAM)-Polyclonal antibody.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Rabbit, Anti-Activated Leukocyte Adhesion Molecule (ALCAM)-Polyclonal antibody
Polyclonal, Antibody, Rabbit, anti
production species: rabbit, antibody to-Activated Leukocyte cellular Adhesion Molecule (activated leukocyte cellular adhesion molecule)-polyclonal (Antibody to)
Alternative to gene target
activated leukocyte cell adhesion molecule, CD166 and MEMD, ALCAM and IDBG-48383 and ENSG00000170017 and 214, protein binding, Cell surfaces, Alcam and IDBG-165765 and ENSMUSG00000022636 and 11658, ALCAM and IDBG-631637 and ENSBTAG00000000088 and 281614