Proteinase K ex. Pichia Pastoris (Type D - Recombinant) for molecular biology & PCR

  • Catalog number
    32753
  • Price
    Please ask
  • Size
    100 mg
  • Reagent properties
    Intermediates and solvents for biotechnological research
  • Chemical CAS Nr
    39450-01-6
  • Group
    PCR, polymerase chain reaction, recombinants
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene
    Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
  • Source
    Recombinants or rec. proteins
  • Gene target
  • Short name
    Proteinase K ex. Pichia Pastoris ( D - Recombinant) for molecular biology & PCR
  • Technique
    Recombinant, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Research sys advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative name
    Proteinase K ex. Pichia Pastoris (classification D - Rec.) to measure molecular biology & PCR test kit
  • Alternative technique
    rec, dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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