Purified vitellogenin from blood plasma of male Red Sea Bream treated with 17ß-Oestradiol
Vitellogenin of Red Sea Bream
Red Sea Bream, no reactivity with Zebra-Killifish and Medaka, cross-reacting with carp in immunoblot
Affinity purified monoclonal antibody in PBS pH 7.4 containing 1% BSA
Product is supplied as
Recommended concentration for use
Immunoblot 0.1-1 µg/ml; ELISA 0.25-2 µg/ml
IB, ELISA, IHC not tested
Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC Mouse IgG (Art. No. PK-6102) or ABC AP Mouse IgG (Art. No. AK-5002) systems are applicable.
Store at -20°C
Go to UniProt/SwissProt
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.