PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit
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Catalog number
K687-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive Assay
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Summary
• Detection method- Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample, in a high-throughput screening (HTS) format.
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Sample Type
Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium etc.
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Features Benefits
• Simple procedure; takes ~ 35-40 minutes to complete the assay • Fast and convenient • Sensitive, efficient and accurate assay
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. The PicoProbe™ G6P Assay Kit is much more sensitive than the G6P Colorimetric Assay Kit for low G6P samples.
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Test
Biovision supplies other types of Assays as 1.
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Conjugate
Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit
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Gene target
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Gene symbol
MIR1302-6, PIRC28, PIRC24, PIRC26, PIRC27, PIRC25, ADCP1
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Short name
™ Glucose-6-Phosphate Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
PicoProbe™ Glucose-6-Phosphate fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-6
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Product images
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