SIRT2 Antibody
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Catalog number6138-100
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PricePlease ask
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Size100 μg
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Antibody TargetSIRT2
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Target Alternative NameSIRT2, SIR2, SIR2L, SIR2L2
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceC terminal peptide of human SIRT2
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AccessionQ8IXJ6
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Gene Id22933
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
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PurificationAffinity purified
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ApplicationWestern blot, Immunohistochemistry
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Positive ControlWestern Blot: Brain tissue extracts
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Application And UsagesWestern blot: 1:500 – 1:2000 dilution, IHC: 1:50 - 1:200 dilution. However, the optimal conditions should be determined individually.
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DescriptionThe sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. This SIRT family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. SIRT2, a human homolog of the yeast SIR2 (silent information regulator2), functions as transcriptional silencing mediator at matingtype loci, telomeres and ribosomal gene clusters. SIRT2 expression increases dramatically during mitosis and is multiply phosphorylated at the G (2)/M transition of the cell cycle. SIRT2 is part of a phosphorylation cascade where it is phosphorylated late in G (2), during M, and into the period of cytokinesis. Inhibition of SIRT2 is reported to rescue alphasynuclein toxicity and modify inclusion morphology in a cellular model of Parkinson's disease.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSIRT2
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Short nameSIRT2 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameSIRT2 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namesirtuin 2
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Synonyms gene
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Synonyms gene name
- sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2
- sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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Pubmed identfication
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Classification
- Sirtuins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data