Anti-SPON2/M spondin/Mindin, ALEXA Fluor 594

• Catalog number
  GENTObs-11064R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  SPON2/M spondin/Mindin
• Specificity
  This antibody reacts specifically with SPON2/M spondin/Mindin
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human SPON2
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The Thrombospondin proteins, Thrombospondins 1-4 and Thrombospondin 5 (also designated COMP), compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Spondin-2, or Mindin, is also designated DIL-1 for its differential expression in cancerous and non-cancerous lung cells. Full-length SPON2 cDNA encodes a 331 amino acid protein with a domain arrangement similar to zebrafish F-Spondin and Mindin-1/Mindin-2: an FS1 domain, an FS2 domain, a hydrophobic signal sequence in the N-terminus and a Thrombospondin type I repeat. Immunoblot analysis demonstrates expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  SPON 2; Dferentially expressed in cancerous and non-cancerous lung cells 1; DIL 1; DIL-1; DIL1; M spondin; Mindin; Spon2; SPON2_HUMAN; Spondin 2; Spondin-2.
• Also known as
  SPON2/M spondin/Mindin Antibody
• Other name
  Anti-SPON2/M spondin/Mindin
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  SPON2/M   spondin/Mindin  
• Gene symbol
  SPON2
• Short name
  Anti-SPON2/M spondin/Mindin
• Technique
  anti-, anti, antibody to, antibodies
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  SPON2/M spondin/Mindin Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  antibodies
• Alternative to gene target
  spondin 2, extracellular matrix protein, DIL-1 and DIL1 and M-SPONDIN and MINDIN, SPON2 and IDBG-6462 and ENSG00000159674 and 100130872,10417, metal ion binding, Extracellular, Spon2 and IDBG-152784 and ENSMUSG00000037379 and 100689, BT.18280 and IDBG-643872 and ENSBTAG00000004261 and 513844

Gene info

• Identity
  HGNC:11253
• Gene
  SPON2
• Long gene name
  spondin 2
• Synonyms gene name
  • spondin 2, extracellular matrix protein
• Synonyms
  • DIL1
• Synonyms name
  • Mindin
  • M-spondin
• GenBank acession
  • AB027466
• Locus
  4p16.3
• Discovery year
  1999-06-18
• Entrez gene record
  10417
• Pubmed identfication
  • 10512675
  • 15094111
• VEGA ID
  OTTHUMG00000089002

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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