Anti Human Stem Cell Kit

• Catalog number
  IMMUNOS TEP-SCK (V50)
• Price
  Please ask
• Size
  50 TEST

• Description
  Anti Human Stem Cell Kit is product reported for use in flow cytometric analysis to identify and determinate hematopoietic stem cells (HSCs), the component of this kit is a monoclonal antibody anti CD34 to rects with the specific antigen of stem cells, a vial of combination of two monoclonal antibodies and a vial of combination of isotype control and monoclonal antibody, counting tubes, 7AAD staining solultion, and a bottle of lysis solution.
• Other flow cytometric products
• Clone
  NA
• Conjugate
  NA
• Application
  Flow Cytometry Method
• Reactivity
  Human
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
• Expiration
  After 1 year
• Storage
  2-8°C, store in dark, do not freeze
• Remark
  For in Vitro Diagnostic Use.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. For cells, cell lines and tissues in culture till half confluency.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

• Gene target
  Stem   Kit  
• Gene symbol
  KIT, KITLG
• Short name
  Anti Stem Kit
• Technique
  anti human, anti, antibody to, anti human antibodies
• Species
  Human, Humans
• Alternative name
  antibody to H. sapiens progenitor cellular reagent
• Alternative technique
  antibodies, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell, stem

Gene info

• Identity
  HGNC:6342
• Gene
  KIT
• Long gene name
  KIT proto-oncogene, receptor tyrosine kinase
• Synonyms gene
  • PBT
• Synonyms gene name
  • piebald trait
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Synonyms
  • CD117
  • SCFR
  • C-Kit
• Synonyms name
  • mast/stem cell growth factor receptor Kit
• GenBank acession
  • S67773
• Locus
  4q12
• Discovery year
  1986-01-01
• Entrez gene record
  3815
• Pubmed identfication
  • 9027509
• RefSeq identity
  • NM_000222
• Classification
  • Receptor tyrosine kinases
  • CD molecules
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000128713
• Locus Specific Databases
  • LRG_307

Gene info

• Identity
  HGNC:6343
• Gene
  KITLG
• Long gene name
  KIT ligand
• Synonyms gene
  • MGF
• Synonyms
  • SCF
  • SF
  • Kitl
  • KL-1
  • FPH2
  • SLF
  • DFNA69
• Synonyms name
  • mast cell growth factor
  • stem cell factor
  • steel factor
  • familial progressive hyperpigmentation 2
• GenBank acession
  • M59964
• Locus
  12q21.32
• Discovery year
  1991-06-04
• Entrez gene record
  4254
• Pubmed identfication
  • 2208279
  • 1707188
  • 19375057
  • 26522471
• RefSeq identity
  • NM_003994
• VEGA ID
  OTTHUMG00000169888
• Locus Specific Databases
  • LRG_1379

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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