DAX-J2 Ratio 580/460

• Catalog number
  16310
• Price
  Please ask
• Size
  1 mg

• Description
  DAX-J2™ Ratio 580/460 is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells in a ratiometric mode under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to induce fluorescence ratio changes at wavelengths of 580 and 460 nm upon NO oxidation. The fluorescence intensities at 580 nm and 460 nm can be detected using the filter sets of Cy3®/TRITC and BD Horizon™ V450/Pacific Blue. These two filter sets are equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Ratio 580/460 has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterse activities are affected by cell health and many other factors. 2). DAX-J2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Ratio 580/460 can be monitored in a ratiometric mode.
• Similar product
• Shipping
  Room Temperature
• Storage
  Freezer
• Shelflife
  12 months
• Molecular Weight
  830.97
• Appearance
  Solid
• Solubility
  Soluble in: DMSO
• Excitation Wavelength
  420/540 nm
• Emission Wavelength
  460/580 nm
• Personal precautions
  Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
• Uses
  Laboratory chemicals *For Research Use Only*
• Technical data
• Data sheet
• Ordering
  To order DAX-J2 Ratio 580/460, please use the Cat. Nr.16310 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for DAX-J2 Ratio 580/460

• Gene target
  DAX-J2   Ratio   580/460  
• Gene symbol
  IGKJ2, TRGJ2
• Short name
  DAX-J2 Ratio 580/460
• Alternative name
  DAX-J2 Ratio 580/460

Gene info

• Identity
  HGNC:5720
• Gene
  IGKJ2
• Long gene name
  immunoglobulin kappa joining 2
• Synonyms
  • J2
• GenBank acession
  • J00242
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28949
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151691

Gene info

• Identity
  HGNC:12278
• Gene
  TRGJ2
• Long gene name
  T cell receptor gamma joining 2
• Synonyms gene
  • TCRGJ2
• Synonyms gene name
  • TCRGJ2
• Synonyms
  • J2
• Synonyms name
  • T-cell receptor gamma joining segment J2
• GenBank acession
  • M12961
• Locus
  7p14.1
• Discovery year
  1989-05-30
• Entrez gene record
  6969
• Pubmed identfication
  • 3003579
• RefSeq identity
  • NG_001336
• Classification
  • T cell receptor gamma locus at 7p14
• VEGA ID
  OTTHUMG00000155217

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