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DAX J2 Ratio 580 460

DAX J2 Ratio 580 460 is available 2 times from Aat labs

16310 | DAX-J2 Ratio 580/460 size: 1 milligram | 433.29 USD

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16310 | DAX-J2 Ratio 580/460 size: 1 mg | 599.88 USD

Catalog number 16310
Supplier aat
Price 599.88 USD
Size 1 mg
Description DAX-J2™ Ratio 580/460 is a new nitric oxide (NO) sensor recently developed by AAT Bioquest. It is a cell permeable reagent that can measure free NO and nitric oxide synthase (NOS) activity in living cells in a ratiometric mode under physiological conditions. Once inside the cell the blocking groups on the DAX-J2 reagent are released to induce fluorescence ratio changes at wavelengths of 580 and 460 nm upon NO oxidation. The fluorescence intensities at 580 nm and 460 nm can be detected using the filter sets of Cy3®/TRITC and BD Horizon™ V450/Pacific Blue. These two filter sets are equipped with most of flow cytometers and fluorescence microscope. DAX-J2 Ratio 580/460 has distinct advantages for NO detection than the popular DAF-2 NO probe: 1). It does not require esterase activity for NO detection. DAF-2 requires intracellular esterases to cleave its acetate groups for detecting NO activity. This esterase dependence often complicates the NO detection since esterse activities are affected by cell health and many other factors. 2). DAX-J2 product exhibits pH-independent fluorescence while DAF-2 has its fluorescence highly affected by pH. 3). DAX-J2 Ratio 580/460 can be monitored in a ratiometric mode.
Tips Our specialists recommend you to follow carefully the pre-registered instructions for DAX-J2 Ratio 580/460
1. Gene info
Identity12278
GeneTRGJ2
Synonyms gene
Synonyms gene name
  • TCRGJ2
Synonyms
GenBank acession
Locus7p14.1
Discovery year1989-05-30
Pubmed identfication
RefSeq identity
Havana BLAST/BLATOTTHUMG00000155217
Product images
    #-#.jpg
Transportation conditions Ambient (Room) temperature
Storage conditions Frozen
Shelf life 1 year
Shipping Room Temperature
Storage Freezer
Shelflife 12 months
Molecular Weight 830.97
Appearance Solid
Solubility Soluble in: DMSO
Excitation Wavelength 420/540 nm
Emission Wavelength 460/580 nm
Personal precautions Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Uses Laboratory chemicals *For Research Use Only*
Ordering To order DAX-J2 Ratio 580/460, please use the Cat. Nr.16310 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Gene targetDAX-J2 Ratio 580/460
Short name DAX-J2 Ratio 580/460
Alternative name DAX-J2 Ratio 580/460
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  • DAX-J2
  • Ratio
  • 580/460
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