Smad2 (Y216) polyclonal antibody

Alternative Name

Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2
Immunogen

Synthetic peptide, corresponding to amino acids 185-234 of Human Smad2.
Swiss Prot

Q15796
Reactivity

Human,Mouse,Rat
Applications

WB IHC IF
Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity

Smad2 (Y216) polyclonal antibody detects endogenous levels of Smad2 protein.
Usage

NA
BiowMW

~ 60 kDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

Smad2 Y216
Short name

Smad2 (Y216) polyclonal antibody
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit
Alternative name

SMAD family member 2 (Y216) polyclonal (antibody to-)
Alternative technique

polyclonals, antibodies
Alternative to gene target

SMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data