HEC1 Antibody

• Catalog number
  3763-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  HEC1
• Target Alternative Name
  HEC, HEC1, HEC-1, Highly Expressed in Cancer 1, KNTC2, Kinetochore Associated 2
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse
• Immunogen Sequence
  Synthetic peptide of human HEC1 (between aa 600-642)
• Accession
  NP_006092.1
• Gene Id
  10403
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation
• Application And Usages
  Western blotting (1:500 – 1:2500) and Immunoprecipitation. HeLa and NIH3T3 cell lysates can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the HEC1 of human and mouse origins. Reactivity to other species has not been tested.
• Description
  Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores. Hec1 is involved in spindle checkpoint signaling. Hec1 is not expressed in terminal differentiated cells. Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus. Hec1 is also found in the late S to M phases of bladder carcinoma cells. In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores. The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithful chromosome segregation. The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  HEC1  
• Gene symbol
  NDC80
• Short name
  HEC1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  HEC1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16909
• Gene
  NDC80
• Long gene name
  NDC80 kinetochore complex component
• Synonyms gene
  • KNTC2
• Synonyms gene name
  • highly expressed in cancer, rich in leucine heptad repeats (yeast)
  • kinetochore associated 2
  • NDC80 kinetochore complex component homolog (S. cerevisiae)
• Synonyms
  • HEC
  • HEC1
  • hsNDC80
  • TID3
• GenBank acession
  • AF017790
• Locus
  18p11.32
• Discovery year
  2004-01-15
• Entrez gene record
  10403
• Pubmed identfication
  • 9315664
  • 12351790
• RefSeq identity
  • NM_006101
• Classification
  • NDC80 kinetochore complex
• VEGA ID
  OTTHUMG00000131483

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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