-
Other name
Mitogen-activated protein kinase 8,JNK-46,Stress-activated protein kinase 1c,SAPK1c,Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,MAPK8,JNK1,PRKM8,SAPK1,SAPK1C
-
Immunogen
Recombinant human Mitogen-activated protein kinase 8 protein (5-300AA)
-
Verified reactivity
Human, Mouse
-
Verified applications
ELISA,WB,IHC,IF. Recommended dilution:WB:1:1000-1:5000,IHC:1:20-1:200,IF:1:50-1:200
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Protein number
P45983
-
Gene number
Please refer to GenBank
-
NCBI number
Please refer to NCBI
-
Purity
Protein G purified, >95%
-
Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
-
Use before
1 year
-
Shipping requirements
Blue ice
-
Estimated production time
3-7 business days
-
Supplementary information
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
-
Notes
For research use only. Not for diagnostic procedures.
-
-
Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
MAPK8
-
Short name
MAPK8 Polyclonal Antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Label
Unconjugated
-
Alternative name
mitogen-activated protein kinase 8 polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
mitogen-activated protein kinase 8, JNK and JNK-46 and JNK1 and JNK1A2 and JNK21B1/2 and PRKM8 and SAPK1 and SAPK1c, MAPK8 and IDBG-73479 and ENSG00000107643 and 5599, transferase activity, nuclei, Mapk8 and IDBG-148417 and ENSMUSG00000021936 and 26419, MAPK8 and IDBG-631635 and ENSBTAG00000007876 and 539941
-
