Glutamine Synthetase, human recombinant
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Catalog numberP1067-10
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PricePlease ask
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Size10 ug
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SynonymsGLNS, GS, PIG43, PIG59, GLUL
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Alternative_namesGLNS, GS, PIG43, PIG59, GLUL
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE> 85%
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AssaySDS-PAGE
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Activity Specifications test methodMeasured by its ability to convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.
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Biological activitySpecific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.
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Results> 2.000 pmol/min/ug
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Unit DefinitionOne unit is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH
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Molecular Weight42 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form descriptionIn 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol 1 mM DTT, 0.1 mM PMSF
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Background InformationGLUL also known as Glutamine synthetase. It is a trimetallic enzyme containing two divalent cation sites and one monovalent cation site per subunit. GLUL is able to regulate intracellular concentrations of glutamate and catalyzes the synthesis of glutamine form glutamate and ammonia. It is ubiquitously expressed in the human and plays a major role for many metabolic pathways such as cell proliferation, inhibition of apoptosis, and cell signaling. Recombinant Human GLUL was expressed in E.coli and purified by using conventional chromatography techniques.
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Amino acid sequenceMTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolASNS, LGSN, QARS1
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Short nameGlutamine Synthetase, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameGlutamine Synthetase, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameasparagine synthetase (glutamine-hydrolyzing)
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Synonyms gene name
- asparagine synthetase
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelengsin, lens protein with glutamine synthetase domain
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Synonyms gene
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Synonyms gene name
- glutamate-ammonia ligase (glutamine synthetase) domain containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-05-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameglutaminyl-tRNA synthetase 1
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Synonyms gene
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Synonyms gene name
- glutaminyl-tRNA synthetase
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Synonyms name
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GenBank acession
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Locus
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Discovery year1994-12-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aminoacyl tRNA synthetases, Class I
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data