Glutamine Synthetase, human recombinant

  • Catalog number
    P1067-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    GLNS, GS, PIG43, PIG59, GLUL
  • Alternative_names
    GLNS, GS, PIG43, PIG59, GLUL
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    > 85%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    Measured by its ability to convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.
  • Biological activity
    Specific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.
  • Results
    > 2.000 pmol/min/ug
  • Unit Definition
    One unit is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH
  • Molecular Weight
    42 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Concentration
    1 mg/ml
  • Appearance
    Liquid
  • Physical form description
    In 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol 1 mM DTT, 0.1 mM PMSF
  • Background Information
    GLUL also known as Glutamine synthetase. It is a trimetallic enzyme containing two divalent cation sites and one monovalent cation site per subunit. GLUL is able to regulate intracellular concentrations of glutamate and catalyzes the synthesis of glutamine form glutamate and ammonia. It is ubiquitously expressed in the human and plays a major role for many metabolic pathways such as cell proliferation, inhibition of apoptosis, and cell signaling. Recombinant Human GLUL was expressed in E.coli and purified by using conventional chromatography techniques.
  • Amino acid sequence
    MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    ASNS, LGSN, QARS1
  • Short name
    Glutamine Synthetase, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    Glutamine Synthetase, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    lengsin, lens protein with glutamine synthetase domain
  • Synonyms gene
  • Synonyms gene name
    • glutamate-ammonia ligase (glutamine synthetase) domain containing 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2003-05-02
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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