Mouse Anti-Rat Kir6.1 Monoclonal IgG2A, Clone S366-60

• Catalog number
  SMC-491D-A594
• Price
  Please ask
• Size
  100 µg

• Clone
  S366-60
• Immunogen
  Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
• Antibody s full description
  Mouse Anti-Rat Kir6.1 Monoclonal IgG2A Antibody, Clone: S366-60: ATTO 594
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  ATP-sensitive inward rectifier potassium channel 8 Antibody, Potassium channel inwardly rectifying subfamily J member 8 Antibody, uKATP-1 Antibody, Kcnj8 Antibody
• Raised in
  Mouse
• Antibody s target
  Kir6.1
• Primary research fields
  Neuroscience, Ion channels, Potassium Channels, Inward-Rectifying Potassium Channels , Cancer
• Brandname
  None
• Antibodies applications
  WB, IHC, ICC/IF
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.1% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~45kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  A 1:100 dilution of SMC-491 was sufficient for detection of Kir6.1 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  Several different potassium channels are known to be involved with electrical signaling in the nervous system. One class is activated by depolarization whereas a second class is not. The latter are referred to as inwardly rectifying K+ channels, and they have a greater tendency to allow potassium to flow into the cell rather than out of it. This asymmetry in potassium ion conductance plays a key role in the excitability of muscle cells and neurons. The protein encoded by this gene is an integral membrane protein and member of the inward rectifier potassium channel family (1-3). This is predominantly detected in fetal and adult hearts, and defects can be associated with J-wave syndromes, a group of heart disorders characterized by early repolarization events (4).
• Bibliography
  1. Zobel C., et al. (2003) J Physiol. 550: 365-372. 2. Panama B.K., McLerie M., and Lopatin A.N. (2007) Am J Physiol Heart Circ Physiol. 293: H3558-H3567. 3. Munoz V., et al. (2007) Heart Rhythm. 4(4): 487-496. 4. Aguilar-Bryan L., et al. (1998) Physiol Rev. 78(1): 227-245.
• Released date
  10-Mar-2015
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_058795.3
• Gene number
  25472
• Protein number
  Q63664
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (SMC-491). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (SMC-491) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Kir6.1 Antibody (D) Composite. Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (SMC-491). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (SMC-491) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa. Mouse Anti-Kir6.1 Antibody [S366-60] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-491) Mouse Anti-Kir6.1 Antibody [S366-60] used in Western Blot (WB) on Rat Brain (SMC-491)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  Kir6   Monoclonal   IgG2A   S366-60  
• Gene symbol
  IGLV4-60, IGHVII-60-1, IGHV3-60, RCC2, OGFR, RNU6-60P, SPINK4, USP32, PPIL2, DYNC2LI1
• Short name
  Mouse Anti- Kir6 1 Monoclonal IgG2A, Clone S366-60
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2A, IgG2a
• Label
  ATTO 594
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat Kir6.1 monoclonal IgG2A, clonality S366-60
• Alternative technique
  murine, antibodies
• Clone name
  S366-60

Gene info

• Identity
  HGNC:5920
• Gene
  IGLV4-60
• Long gene name
  immunoglobulin lambda variable 4-60
• GenBank acession
  • Z73667
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28785
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000150851

Gene info

• Identity
  HGNC:5686
• Gene
  IGHVII-60-1
• Long gene name
  immunoglobulin heavy variable (II)-60-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-60-1
  • immunoglobulin heavy variable (II)-60-1 pseudogene
• Synonyms
  • IGHV(II)-60-1
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28361
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151951

Gene info

• Identity
  HGNC:5614
• Gene
  IGHV3-60
• Long gene name
  immunoglobulin heavy variable 3-60 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 3-60
  • immunoglobulin heavy variable 3-60 pseudogene
• GenBank acession
  • M29813
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28417
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151956

Gene info

• Identity
  HGNC:30297
• Gene
  RCC2
• Long gene name
  regulator of chromosome condensation 2
• Synonyms
  • TD-60
• Locus
  1p36.13
• Discovery year
  2005-06-07
• Entrez gene record
  55920
• Pubmed identfication
  • 10819331
  • 12919680
• RefSeq identity
  • NM_018715
• VEGA ID
  OTTHUMG00000002513

Gene info

• Identity
  HGNC:15768
• Gene
  OGFR
• Long gene name
  opioid growth factor receptor
• Synonyms
  • 7-60
• GenBank acession
  • AF109134
• Locus
  20q13.33
• Discovery year
  2001-05-31
• Entrez gene record
  11054
• Pubmed identfication
  • 10677613
  • 26687326
• VEGA ID
  OTTHUMG00000032937

Gene info

• Identity
  HGNC:42550
• Gene
  RNU6-60P
• Long gene name
  RNA, U6 small nuclear 60, pseudogene
• Synonyms gene
  • RNU6-60
• Synonyms gene name
  • RNA, U6 small nuclear 60
• Locus
  13q14.2
• Discovery year
  2011-08-08
• Entrez gene record
  106480750

Gene info

• Identity
  HGNC:16646
• Gene
  SPINK4
• Long gene name
  serine peptidase inhibitor Kazal type 4
• Synonyms gene name
  • serine protease inhibitor, Kazal type 4
• Synonyms
  • PEC-60
  • MGC133107
• GenBank acession
  • AF048700
• Locus
  9p13.3
• Discovery year
  2001-09-21
• Entrez gene record
  27290
• Pubmed identfication
  • 1400298
  • 17333166
• RefSeq identity
  • NM_014471
• Classification
  • Serine peptidase inhibitors, Kazal type
• VEGA ID
  OTTHUMG00000019762

Gene info

• Identity
  HGNC:19143
• Gene
  USP32
• Long gene name
  ubiquitin specific peptidase 32
• Synonyms gene name
  • ubiquitin specific protease 32
• Synonyms
  • NY-REN-60
  • USP10
• GenBank acession
  • AF533230
• Locus
  17q23.3
• Discovery year
  2003-05-29
• Entrez gene record
  84669
• Pubmed identfication
  • 12838346
• RefSeq identity
  • NM_032582
• Classification
  • EF-hand domain containing
  • Ubiquitin specific peptidases
• VEGA ID
  OTTHUMG00000180001

Gene info

• Identity
  HGNC:9261
• Gene
  PPIL2
• Long gene name
  peptidylprolyl isomerase like 2
• Synonyms gene name
  • peptidylprolyl isomerase (cyclophilin)-like 2
• Synonyms
  • UBOX7
  • CYC4
  • Cyp-60
• Synonyms name
  • U-box domain containing 7
  • peptidyl-prolyl cis-trans isomerase-like 2
• Locus
  22q11.21
• Discovery year
  1999-10-29
• Entrez gene record
  23759
• Pubmed identfication
  • 10591208
• Classification
  • Spliceosomal Bact complex
  • U-box domain containing
  • Cyclophilin peptidylprolyl isomerases
• VEGA ID
  OTTHUMG00000191763

Gene info

• Identity
  HGNC:24595
• Gene
  DYNC2LI1
• Long gene name
  dynein cytoplasmic 2 light intermediate chain 1
• Synonyms
  • D2LIC
  • LIC3
  • CGI-60
  • DKFZP564A033
• Locus
  2p21
• Discovery year
  2005-11-29
• Entrez gene record
  51626
• Pubmed identfication
  • 10810093
  • 11907264
• RefSeq identity
  • NM_016008
• Classification
  • Dynein 2 complex subunits
• VEGA ID
  OTTHUMG00000128656

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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