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Rabbit Anti Rat SOD Mn Polyclonal

Rabbit Anti Rat SOD Mn Polyclonal is available 20 times from Stressmark antibodies labs

SPC-117D-ALP | Rabbit Anti-Rat SOD (Mn) Polyclonal: Alkaline Phosphatase size: 100 µg | 446.86 USD

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SPC-117D-APC | Rabbit Anti-Rat SOD (Mn) Polyclonal: APC size: 100 µg | 452.39 USD

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SPC-117D-A390 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 390 size: 100 µg | 454.61 USD

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SPC-117D-A488 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 488 size: 100 µg | 453.50 USD

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SPC-117D-A565 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 565 size: 100 µg | 453.50 USD

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SPC-117D-A594 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 594 size: 100 µg | 453.50 USD

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SPC-117D-A633 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 633 size: 100 µg | 453.50 USD

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SPC-117D-A655 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 655 size: 100 µg | 453.50 USD

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SPC-117D-A680 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 680 size: 100 µg | 453.50 USD

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SPC-117D-A700 | Rabbit Anti-Rat SOD (Mn) Polyclonal: ATTO 700 size: 100 µg | 453.50 USD

Catalog number SPC-117D-A700
Price 453.50 USD
Size 100 µg
Net weight (g) 0.1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name Oryctolagus cuniculus,Rattus norvegicus
Gene targetSOD ATTO 700
Short name Rabbit Anti- SOD (Mn) Polyclonal: ATTO 700
Technique Polyclonal, Rabbit, anti
Alternative name production species: rabbit antibody to-Rat antioxidant enzyme (Mn) polyclonal: ATTO 700
1. Gene info
Identity 11181
Gene SOD3
Long gene name superoxide dismutase 3
Synonyms gene name
  • superoxide dismutase 3, extracellular
Synonyms
  • EC-SOD
Locus 4p15.2
Discovery year 1988-05-08
Entrez gene record 6649
Havana BLAST/BLAT OTTHUMG00000128565
MeSH Data
Name Blotting, Western
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Rat#.png

SPC-117D-BI | Rabbit Anti-Rat SOD (Mn) Polyclonal: Biotin size: 100 µg | 449.08 USD

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SPC-117D-FITC | Rabbit Anti-Rat SOD (Mn) Polyclonal: FITC size: 100 µg | 444.65 USD

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SPC-117D-HRP | Rabbit Anti-Rat SOD (Mn) Polyclonal: HRP size: 100 µg | 440.23 USD

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SPC-117D-P594 | Rabbit Anti-Rat SOD (Mn) Polyclonal: PE/ATTO 594 size: 100 µg | 461.24 USD

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SPC-117D-PCP | Rabbit Anti-Rat SOD (Mn) Polyclonal: PerCP size: 100 µg | 452.39 USD

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SPC-117D-RPE | Rabbit Anti-Rat SOD (Mn) Polyclonal: RPE size: 100 µg | 450.18 USD

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SPC-117D-STR | Rabbit Anti-Rat SOD (Mn) Polyclonal: Streptavidin size: 100 µg | 451.29 USD

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SPC-117C | Rabbit Anti-Rat SOD (Mn) Polyclonal: Unconjugated size: 25 µg | 225.64 USD

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SPC-117S | Rabbit Anti-Rat SOD (Mn) Polyclonal: Unconjugated size: 12 µg | 55.31 USD

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SPC-117D | Rabbit Anti-Rat SOD (Mn) Polyclonal: Unconjugated size: 100 µg | 401.51 USD

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Immunogen Rat Mn SOD
Antibody's target Rat SOD2
Antibody's full description Rabbit Anti-Rat SOD (Mn) Polyclonal
Primary research fields Cancer, Oxidative Stress, Cell Signaling, Neuroscience, Neurodegeneration, Cardiovascular System
Antibody's category Polyclonal Antibodies
Antibody's other name Manganese SOD Antibody, IPO B Antibody, Mn SOD Antibody, SOD2 Antibody
Verified applications WB, IHC, ICC/IF, IP, ELISA
Raised in Rabbit
Antibody's reactivity Human, Rat, Mouse, Bovine, Dog, Chicken, Fruit Fly (Drosophila melanogaster), Guinea Pig (Cavia porcellus), Pig, Hamster, Monkey, Rabbit, Sheep, African clawed frog (Xenopus laevis) , Squirrel, Invertebrate, Sea squirt (Ciona intestinalis)
Antibody's recommended dilutions for use WB (1:5000), IHC (1:100), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Antibody's purified from Protein A Purified
Recommended buffer for storage PBS pH7.4, 50% glycerol, 0.09% sodium azide
Antibody's concentration 1 mg/ml
Antibody's specificity Detects ~25kDa.
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Certificate of analysis 0.5 µg/ml of SPC-117 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Antibody in cell Mitochondrion, Mitochondrion Matrix
Tissue specificity See included datasheet or contact our support service.
Scientific context Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Bibliography 1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
Released date 1/Jun/2007
NCBI number NP_058747.1
Gene number 24787
Protein number P07895
PubMed number 26458773|23067137|23067137|22086848|19272187
Tested applications Immunohistochemistry|Immunohistochemistry |Western Blot|Western Blot|Western Blot
Tested reactivity Mouse|Ciona intestinalis (Sea squirt) |Ciona intestinalis (Sea squirt) |Squirrel|Rat
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Representative figure linkmore | details | see here | read more
Representative figure legend Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody (SPC-117). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (SPC-117) at 1:120 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite. | Western blot analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. | Immunohistochemistry analysis using Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody (SPC-117) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Mitochondrion matrix. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Mn) Polyclonal Antibody (SPC-117). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (SPC-117) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Mn) Antibody. (C) Composite. Rabbit Anti-SOD (Mn) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-117) | Rabbit Anti-SOD2 Antibody used in Western blot (WB) on Rat Tissue lysates (SPC-117) | Rabbit Anti-SOD2 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-117) | Rabbit Anti-SOD (Mn) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HeLa Cells (SPC-117)
Warning information Non-hazardous
Country of production Canada
Total weight (kg) 1.4
Stock availabilit In Stock
Host Rabbit
Species Rat
Alternative technique polyclonals
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