Trypsin - EDTA 1X Lyophilised w/ Sodium Chloride - 100g
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Catalog number
PM-T1717/100
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Price
Please ask
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Size
5X100g
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Description
Trypsin - EDTA 1X Lyophilised w/ Sodium Chloride - 100g is used for the dissociation and disaggregation of mammalian cells and tissues.
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Shipping conditions
-20°C or dry ice
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Storage
-20 ° C
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Shelflife
24 months
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State
Frozen
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Chemical description
NA
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Appearance
NA
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Density at 20 ºC
NA
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Relative density at 20 ºC
NA
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PH
NA
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Osmolality
NA
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Cell growth test
NA
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Sterility
Sterilizing Filtration
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Used in biotechnology. For professional user/industrial user only.
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Data sheet
Contact us to request a Data sheet
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Ordering
To order Trypsin - EDTA 1X Lyophilised w/ Sodium Chloride - 100g, please use the Cat. Nr.PM-T1717/100 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Trypsin - EDTA 1X Lyophilised w/ Sodium Chloride - 100g
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Buffer
Plasmas are treated with K2, K3 or Na EDTA disodium chelator at pH8. The Ethylene-diamine-tetra acetic acid with Cas nr 60-00-4 is called Edetic acid. The chemical formula is C10H16N2O8. As anticoagulant GENTAUR also uses heparin and sodium citrate to produce plasmas.
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Additional description
Lyphilized ultra pure white powder needs to be reconstututed in PBS, Sodium Phosphate or RPMI and sterile Water supplemented with 0% glycerol or gelatin in as high as possible concentration aliquots to prevent post reconstitution degradation. Aliquoting with 25 mM HEPES-KOH, pH 7.2 and 1 mM DTT and 10% weight on volume glycerol is suitable for storage of the reconstituted powder. The solution can be stored for short term at +4 C 5 days and long term storage at - 85C. Freeze thaw cycles need to be omitted to maintain the protein activity. A typical loss of 15 % in each freeze thaw cycle can be expected.
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