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Also known as
CD147
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Other names
basigin isoform 2; Basigin; basigin; gp 42; HT7 antigen; neurothelin; 5A11/Basigin; membrane glycoprotein gp42; basic immunoglobulin superfamily; basigin; Basic immunoglobulin superfamily; HT7 antigen; Membrane glycoprotein gp42
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD147
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Gene name synonims
N/A
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Other gene names
Bsg; Bsg; HT-7; CD147; EMMPRIN; AI115436; AI325119; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
OX114
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Host organism
Rat (Rattus norvegicus)
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD147 This item recognises mouse CD147 (also known as basigin). CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases. _x000D__x000D_OX-114 recognises both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains. _x000D__x000D_This antibody is routinely tested in flow cytometry on mouse splenocytes. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Purified (Purified IgG - liquid)
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Concentration
IgG concentration 1.0 mg/ml
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Immunohistology - Frozen (IHC), Western Blot (WB), Flow Cytometry (FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translation
anticorps
