PDK, Active, M. rosea Recombinant

• Catalog number
  P1161-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Pyruvate Phosphate Dikinase, Pyruvate, orthophosphate dikinase
• Alternative_names
  Pyruvate Phosphate Dikinase, Pyruvate, orthophosphate dikinase
• Description
  Plays an important role in the glycolytic pathway
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  > 90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by the conversion of 1 umole of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate at 37ºC and pH 7.0.
• Biological activity
  Specific activity is ≥ 0.8 U/mg
• Results
  ≥ 0.8 U/mg
• Unit Definition
  One unit of enzyme converts 1 umole of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate at 37ºC and pH 7.0.
• Molecular Weight
  95.5 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  Proprietary buffer
• Background Information
  Pyruvate phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in certain microorganisms and plants. PPDK catalyzes the interconversion of AMP, PPi, and phosphoenolpyruvate (PEP) to ATP, Pi, and pyruvate monophosphate. PPDK plays an important role in the glycolytic pathway and in organisms where pyruvate kinase (PK) is absent. PPDK functions in the process of ATP synthesis. BioVision’s active PPDK is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PDK   Active   rosea  
• Short name
  PDK, Active, M. rosea Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  PDK, functionnal, M. rosea Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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