N6-Methyladenosine Antibody
-
Catalog number6653-100
-
PricePlease ask
-
Size100 μg
-
-
Antibody TargetN6-Methyl Adenosine
-
Target Alternative Namem6A
-
Antibody TypePolyclonal
-
Species ReactivitiesMammalian samples
-
Immunogen SequenceThis antibody is generated from rabbits immunized with a BSA conjugated N6-Methyladenosine (BV-N13-14).
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration1 mg/ml
-
Formulation100 µg (1 mg/ml) in PBS (prepared using DEPC-treated water) with 0.09% (W/V) sodium azide.
-
PurificationProtein A and immunogen affinity purified antibody
-
ApplicationWestern blot, Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
-
Positive ControlWestern blot: N6-Methyladenosine conjugated with BSA, Adenosine conjugated with BSA
-
Application And UsagesWestern blot: 1-4 µg/ml. Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
-
DescriptionN6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolYTHDF3, METTL3, METTL16
-
Short nameN6-Methyladenosine Antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameN6-Methyladenosine (antibody to-)
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameYTH N6-methyladenosine RNA binding protein 3
-
Synonyms gene name
- YTH domain family 3
- YTH domain family, member 3
- YTH N(6)-methyladenosine RNA binding protein 3
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2004-06-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemethyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit
-
Synonyms gene name
- methyltransferase like 3
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2003-01-14
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- m6A methyltransferase complex
- 7BS N6-adenosine DNA/RNA methyltransferases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemethyltransferase 16, N6-methyladenosine
-
Synonyms gene
-
Synonyms gene name
- methyltransferase 10 domain containing
- methyltransferase like 16
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2005-12-19
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- 7BS N6-adenosine DNA/RNA methyltransferases
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data