MOUSE Anti-CHICKEN CD45:FITC Antibody

  • Catalog number
    GEN210974
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    CD45
  • Other names
    N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    CD45
  • Gene name synonims
    N/A
  • Other gene names
    N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2a
  • Clone
    UM16-6
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    CD45 This item recognizes chicken CD45 (leucocyte common antigen), a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. _x000D__x000D_CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. Studies have indicated that dephosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. _x000D__x000D_Investigations into the properties of CD45 in chicken models are limited, but there is evidence of the existence of an additional cysteine residue near the transmembrane region. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
  • Concentration
    IgG concentration 0.1 miligram per 1 mililiter
  • Storage and shipping
    Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
  • Tested applications
    Flow Cytometry (FACS)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
    CD45  
  • Gene symbol
    PTPRCAP, PTPRC
  • Short name
    MOUSE Anti- CD45:FITC Antibody
  • Technique
    Antibody, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
  • Host
    mouse
  • Label
    FITC
  • Species
    chicken, Chickens, Mouses
  • Alternative name
    Anti-MOUSE ANTI CHICKEN CD45:FITC
  • Alternative technique
    antibodies, murine, fluorescine
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The number of CD4-POSITIVE T-LYMPHOCYTES per unit volume of BLOOD. Determination requires the use of a fluorescence-activated flow cytometer.
  • Tree numbers
    • E01.370.225.500.195.107.595.500.150
    • E01.370.225.625.107.595.500.150
    • E05.200.500.195.107.595.500.150
    • E05.200.625.107.595.500.150
    • E05.242.195.107.595.500.150
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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