Toluidine Blue DNA Agar

Description

Toluidine Blue DNA Agar is used to detect thermostable deoxyribonuclease activity.
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Deoxyribonucleic acid (DNA) 0.300 Calcium chloride 0.0055 Sodium chloride 10.000 Toluidine blue 0.083 Tris (hydroxymethyl) amino methane 6.100 Agar 10.000 Final pH ( at 25°C) 9.0±0.2
Quantity required per litre

26,48 g
Appearance

Light yellow to light grey homogeneous free flowing powder
Colour and Clarity of prepared medium

Blue coloured clear to slightly opalescent gel forms in Petri plates
Cultural response

18 hrs old Beain Heart Infusion broth culture is heated in boiling water bath for 15 minutes and studied for thermonuclease activity. 5 mm cut wells are cut in agar plates and is filled with 25-30µl of this culture and incubated at 35-37°C for 4 hrs (or it can also be incubated at 50°C for 2 hrs) and observed for results.
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Properties

Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.
Conjugation

Blue Substrate

Name

Electrophoresis, Agar Gel
Concept

Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Tree numbers
- E05.196.401.153
- E05.301.300.100
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data