Heparin Sepharose Column

  • Catalog number
    6554-5
  • Price
    Please ask
  • Size
    5ml
  • Description
    Columns with high binding capacity beads for affinity purification of heparin binding proteins. Heparin-Sepharose is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.
  • Product Highlights
    • CONTENTS: Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in 20 % Ethanol/dH2O. > 2.5 mg heparin per ml Sepharose beads. • FEATURES: Heparin-beads have been widely used in affinity purification of various heparin-binding proteins or ligand, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and other ligands. BioVison’s Heparin-Sepharose is designed for the purpose of purification of these kinds of proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This Heparin-Sepharose formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10. • APPLICATIONS: Purification of heparin-binding proteins, enzymes or other ligands.
  • Storage Temp
    +4°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Gene target
  • Short name
    Heparin Sepharose Column
  • Alternative name
    Heparin Sepharose Column
MeSH Data
  • Name
  • Concept
    Scope note: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
  • Tree numbers
    • E05.196.181
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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