Mouse, Anti-FLAG Octapeptide (FLAG) Tag Monoclonal Antibody-Monoclonal antibody

  • Catalog number
    TAX159Ge22-1mg
  • Price
    Please ask
  • Size
    1mg
  • Organism Species
    Pan-species (General)
  • Source
    Monoclonal antibody preparation
  • Purification
    Protein A + Protein G affinity chromatography
  • Buffer Formulation
    0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • Item Name
    FLAG Octapeptide
  • Immunogen
    CPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
  • Image number
    -
  • Species reactivity
    Pan-species
  • Sequence of immunogen
    -
  • Aplication
    WB; IHC; ICC; IP.
  • Clonality
    Mouse monoclonal
  • Concentration
    1mg/ml
  • Alternative Names
    FLAG epitope; FLAG-Tag
  • Applicable Secondary Antibody
    SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
  • Delivery condition
    4℃ with ice bags
  • Storage instructions
    Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse, Anti- Octapeptide ( ) Tag Monoclonal Antibody-Monoclonal antibody
  • Technique
    Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, Tags
  • Host
    Mouse
  • Label
    Flag
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse, Antibody toFLAG Octapeptide (FLAG) detection labelled monoclonal (antibody to-)-monoclonal (antibody to-)
  • Alternative technique
    antibodies, murine
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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