Clonality Mouse monoclonal
Applicable Secondary Antibody SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
Delivery condition 4℃ with ice bags
Source Monoclonal antibody preparation
Sequence of immunogen -
Image number -
Immunogen CPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
Species reactivity Pan-species
Purification Protein A + Protein G affinity chromatography
Organism Species Pan-species (General)
Alternative Names FLAG epitope; FLAG-Tag
Storage instructions Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
Item Name FLAG Octapeptide
Buffer Formulation 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Aplication WB; IHC; ICC; IP.
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus
French translation anticorps
Short name Mouse, Anti- Octapeptide ( ) Tag Monoclonal Antibody-Monoclonal antibody
Technique Antibody, Mouse, anti
Alternative name Mouse, antibody to-FLAG Octapeptide (FLAG) detection labelled monoclonal (Antibody to)-monoclonal (Antibody to)
Alternative technique antibodies