HGF human ELISA Kit

HGF human ELISA Kit is available 2 times from Biovision labs

K4781-100 | HGF (human) ELISA Kit size: 100 assays | 1,104.62 USD

Catalog number K4781-100

Supplier Biovision

Price 1,104.62 USD

Size 100 assays

Buy 3 for 3 026.27 USD (-8.68%) Buy 5 for 5 016.39 USD (-9.17%) Buy 10 for 9 988.64 USD (-9.57%)

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Kit's description BioVision’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of HGF is typically less than 3 pg/ml. Detection Range: 3 pg/ml – 2,000 pg/ml. The intra-Assay reproducibility is CV

Kit's other name Hepatocyte Growth Factor (human) ELISA Kit

Contents • HGF Microplate (Item A) coated with anti-human HGF, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human HGF) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) • Detection Antibody HGF (Item F), biotinylated anti-human HGF (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 400x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid

Samples tested • Serum & plasma (EDTA & citrate) • Cell culture supernatants • Urine

Gene targetHGF

Short name HGF ( ) ELISA Kit

Alternative name hepatocyte growth factor (hepapoietin A; scatter factor) (H. sapiens) Enzyme-linked immunosorbent assay reagent

1. Gene info

Identity 11187

Gene SOS1

Long gene name SOS Ras/Rac guanine nucleotide exchange factor 1

Synonyms gene

GINGF

Synonyms gene name

gingival fibromatosis, hereditary, 1
son of sevenless homolog 1 (Drosophila)

Synonyms

GenBank acession

L13857

Locus 2p22.1

Discovery year 1993-10-27

Entrez gene record 6654

Pubmed identfication

8276400
10995566

RefSeq identity

NM_005633

Classification

Dbl family Rho GEFs
Pleckstrin homology domain containing

Havana BLAST/BLAT OTTHUMG00000102109

Locus Specific Databases

LRG_754

2. Gene info

Identity 4894

Gene HGFAC

Long gene name HGF activator

Synonyms

HGFAP
HGFA

GenBank acession

D14012

Locus 4p16.3

Discovery year 1998-06-25

Entrez gene record 3083

Pubmed identfication

7683665
8226803

Havana BLAST/BLAT OTTHUMG00000090281

Product images

K4782-100 | HGF (human) ELISA Kit (For Lysates) size: 100 assays | 1,104.62 USD

Highlights • Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

Kit's benefits • Easy, convenient and time-saving method to assay HGF. • The minimum detectable concentration of HGF is less than 3 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF).

Category HGF

Storage condition -20°C

Shipping condition gel pack

Maximum time can be stored 12 months

Species reactivity human

Applications Quantitative protein detection, establishing normal range, validation of antibody array results

Kit's specifications

Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Technique ELISA kit, ELISA

Host antigen assay ELISA kit 1 Kit (96 Wells)

Species Human

Alternative technique elisas

Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, hepatocyte growth factor (hepapoietin A; scatter factor), DFNB39 and F-TCF and HGFB and HPTA and SF, HGF and IDBG-24150 and ENSG00000019991 and 3082, protein heterodimerization activity, Extracellular, Hgf and IDBG-136449 and ENSMUSG00000028864 and 15234, HGF and IDBG-631194 and ENSBTAG00000017664 and 282879