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HGF human ELISA Kit

HGF human ELISA Kit is available 2 times from Biovision labs

K4781-100 | HGF (human) ELISA Kit size: 100 assays | 1,104.62 USD

Catalog number K4781-100
Supplier Biovision
Price 1,104.62 USD
Size 100 assays
Kit's description BioVision’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of HGF is typically less than 3 pg/ml. Detection Range: 3 pg/ml – 2,000 pg/ml. The intra-Assay reproducibility is CV
Kit's other name Hepatocyte Growth Factor (human) ELISA Kit
Contents • HGF Microplate (Item A) coated with anti-human HGF, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human HGF) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) • Detection Antibody HGF (Item F), biotinylated anti-human HGF (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 400x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid
Samples tested • Serum & plasma (EDTA & citrate) • Cell culture supernatants • Urine
Gene targetHGF
Short name HGF ( ) ELISA Kit
Alternative name hepatocyte growth factor (hepapoietin A; scatter factor) (H. sapiens) Enzyme-linked immunosorbent assay reagent
1. Gene info
Identity 11187
Gene SOS1
Long gene name SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms gene
  • GINGF
Synonyms gene name
  • gingival fibromatosis, hereditary, 1
  • son of sevenless homolog 1 (Drosophila)
Synonyms
  • HGF
  • GF1
GenBank acession
  • L13857
Locus 2p22.1
Discovery year 1993-10-27
Entrez gene record 6654
Pubmed identfication
  • 8276400
  • 10995566
RefSeq identity
  • NM_005633
Classification
  • Dbl family Rho GEFs
  • Pleckstrin homology domain containing
Havana BLAST/BLAT OTTHUMG00000102109
Locus Specific Databases
  • LRG_754
2. Gene info
Identity 4894
Gene HGFAC
Long gene name HGF activator
Synonyms
  • HGFAP
  • HGFA
GenBank acession
  • D14012
Locus 4p16.3
Discovery year 1998-06-25
Entrez gene record 3083
Pubmed identfication
  • 7683665
  • 8226803
Havana BLAST/BLAT OTTHUMG00000090281
Product images
    #Human#.jpg#0#.jpegHGF (human) ELISA Kit

K4782-100 | HGF (human) ELISA Kit (For Lysates) size: 100 assays | 1,104.62 USD

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Highlights • Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits • Easy, convenient and time-saving method to assay HGF. • The minimum detectable concentration of HGF is less than 3 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF).
Category HGF
Storage condition -20°C
Shipping condition gel pack
Maximum time can be stored 12 months
Species reactivity human
Applications Quantitative protein detection, establishing normal range, validation of antibody array results
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, hepatocyte growth factor (hepapoietin A; scatter factor), DFNB39 and F-TCF and HGFB and HPTA and SF, HGF and IDBG-24150 and ENSG00000019991 and 3082, protein heterodimerization activity, Extracellular, Hgf and IDBG-136449 and ENSMUSG00000028864 and 15234, HGF and IDBG-631194 and ENSBTAG00000017664 and 282879
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HGF ( ) ELISA Kit | Technique alternative | 01012613908
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