Human CellExp™ TPO, Human Recombinant
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Catalog number6483-10
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PricePlease ask
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Size10 µg
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SynonymsHuman Cellexp Human Recombinant TPO
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Alternative_namesMegakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.
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DescriptionStimulates the proliferation and maturation of megakaryocytes
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RecombinantYes
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SourceHuman 293 cell expressed
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Purity by SDS PAGE> 95%
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AssaySDS-PAGE
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Endotoxin Level< 1.0 EU per 1 ug of protein
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Biological activityED₅₀ is typically 0.5 to 5 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of human M07e cells
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Results0.5 to 5 ng/ml
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Molecular Weight80 to 85 kDa, monomer, glycosylated
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized
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Physical form descriptionLyophilized in PBS.
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Reconstitution InstructionsReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
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Background InformationTPO (Thrombopoietin, also known as Mpl-ligand, MGDF), a glycoprotein hormone produced mainly by the liver and the kidney, stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolTPO, THPO
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Short nameCellExp™ TPO, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ TPO, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namethyroid peroxidase
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Synonyms
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- Sushi domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namethrombopoietin
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Synonyms gene
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-11-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data