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Synonyms
CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
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Alternative_names
CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
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Description
Modulates receptor-ligand interactions to enhance leukocyte activation
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 ug/ml (100 ul/well) can bind human CD48. The EC50 of human CD48 is 0.39 ug/ml.
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Results
Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 ug/ml (100 ul/well) can bind human CD48. The EC50 of human CD48 is 0.39 ug/ml.
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Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 23.1 kDa. The predicted N-terminus is Cys 22. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation.
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background Information
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contain two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD244, GSTT1-AS1
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Short name
CellExp™ CD244 / 2B4 / SLAMF4, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Humans
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Alternative name
H. sapiens CellExp™ CD244 molecule, natural killer cellular receptor 2B4 / 2B4 / SLAMF4, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
CD244 molecule, natural killer cell receptor 2B4, CD244 and IDBG-104114 and ENSG00000122223 and 51744, protein binding, Cell surfaces, Cd244 and IDBG-204694 and ENSMUSG00000004709 and 18106, CD244 and IDBG-630400 and ENSBTAG00000002951 and 513468
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