anti-Cytokeratin5 Antibody [3E2F1]

• Catalog number
  32-142
• Price
  Please ask
• Size
  0.1 mL

• Gene info
  CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
• Gene names
  58 kDa cytokeratin, CK-5, K5, CK5, DDD, DDD1, EBS2, KRT5A
• Antibodie s label
  unlabelled serum
• Ab raised in
  Mouse
• Type
  Monoclonal
• Monoclonal
  3E2F1
• Immunoglobulin
  IgG1
• Antigen
  Ni-NTA purified truncated recombinant CK5-Trx-His expressed in E. Coli strain BL21 (DE3).
• Immuno application
  ELISA, Western Blot, immuno-histo-chemistry
• Antibody advice
  Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
• Suspention pH azide
  Ascitic fluid containing 0.03% sodium azide.
• Keep at
  Cytokeratin 5 monoclonal antibody can be stored at -20 grades C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Works with
  Human
• Omim nr
  143811411
• Ncbi nr
  P13647
• NCBI GENE nr
  3852
• GENE symbol
  KRT5
• Antigene name
  keratin 5
• Source
  Homo sapiens
• Protein nr
  P13647
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Cytokeratin5   [3E2F1]  
• Short name
  anti-Cytokeratin5 Antibody [3E2F1]
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toCytokeratin5 (antibody to-) [3E2F1]
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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