ENA-78, human recombinant

• Catalog number
  4025-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  human ENA-78, Epithelial neutrophil-activating protein 78
• Alternative_names
  Small inducible cytokine B5, CXCL5, Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide, ENA-78, ENA-78(1-78), chemokine (C-X-C motif) ligand 5, SCYB5,
• Description
  A chemokine and activator for neutrophils
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  The human ENA-78 produces chemotaxis over a wide concentration range in an assay using human peripheral blood neutrophils. Significant chemotaxis was achieved using a concentration of 50 ng/ml.
• Results
  50 ng/ ml
• Molecular Weight
  8.0 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized with no additives. The protein may appear as a haze or film which is difficult to see at the bottom of the vial.
• Reconstitution Instructions
  Reconstitute in H₂O to a concentration of 0.1-1 µg/µl. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.
• Background Information
  ENA-78 (Epithelial Neutrophil Activating Peptide-78) is a recently discovered chemoattractant and activator for neutrophils, belonging to the IL-8 subgroup of the C-X-C family of chemokines. The human ENA-78 is an 8.0 kDa protein containing 74 amino acid residues.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ENA-78  
• Gene symbol
  CXCL5, IGHV5-78, IGHVII-78-1, RNU6-78P, APH1A
• Short name
  ENA-78, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  ENA-78, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:10642
• Gene
  CXCL5
• Long gene name
  C-X-C motif chemokine ligand 5
• Synonyms gene
  • SCYB5
• Synonyms gene name
  • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
  • chemokine (C-X-C motif) ligand 5
• Synonyms
  • ENA-78
• GenBank acession
  • X78686
• Locus
  4q13.3
• Discovery year
  1996-09-03
• Entrez gene record
  6374
• Pubmed identfication
  • 7929219
• RefSeq identity
  • NM_002994
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000160871

Gene info

• Identity
  HGNC:5660
• Gene
  IGHV5-78
• Long gene name
  immunoglobulin heavy variable 5-78 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 5-78
  • immunoglobulin heavy variable 5-78 pseudogene
• GenBank acession
  • X92213
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28387
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151873

Gene info

• Identity
  HGNC:5691
• Gene
  IGHVII-78-1
• Long gene name
  immunoglobulin heavy variable (II)-78-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-78-1
  • immunoglobulin heavy variable (II)-78-1 pseudogene
• Synonyms
  • IGHV(II)-78-1
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28356
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151863

Gene info

• Identity
  HGNC:42568
• Gene
  RNU6-78P
• Long gene name
  RNA, U6 small nuclear 78, pseudogene
• Synonyms gene
  • RNU6-78
• Synonyms gene name
  • RNA, U6 small nuclear 78
• Locus
  13q12.13
• Discovery year
  2011-08-08
• Entrez gene record
  100873778
• RefSeq identity
  • NR_046944

Gene info

• Identity
  HGNC:29509
• Gene
  APH1A
• Long gene name
  aph-1 homolog A, gamma-secretase subunit
• Synonyms gene name
  • anterior pharynx defective 1 homolog A (C. elegans)
  • APH1A gamma secretase subunit
• Synonyms
  • APH-1A
  • CGI-78
• GenBank acession
  • AF151835
• Locus
  1q21.2
• Discovery year
  2005-02-08
• Entrez gene record
  51107
• Pubmed identfication
  • 10810093
  • 12110170
• RefSeq identity
  • NM_016022
• VEGA ID
  OTTHUMG00000012545

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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