CD11, CD18 (FITC)
-
Catalog numberGEN631540
-
PricePlease ask
-
Size100ug
-
-
Also known asCD11, CD18
-
Other namesCD11b=leucocyte integrin {N-terminal}; N/A
-
CategoryAntibodies
-
SubcategoryMnoclonal antibodies
-
Gene nameN/A
-
Gene name synonimsN/A
-
Other gene namesN/A
-
ClonalityMonoclonal
-
Immunoglobulin isotypeIgG2c
-
Clone10B561
-
Host organismRat (Rattus norvegicus)
-
Source organismCanine
-
Species reactivityCanine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivityRecognizes canine CD11/18 group between 90/180kD.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification methodAffinity Purified by Protein G affinity chromatography.
-
Form AppearanceSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
-
ConcentrationN/A
-
Storage and shippingStore the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
-
Tested applicationsFlow Cytometry (FC/FACS)
-
PropertiesThis MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
Gene target
-
Gene symbolITGB2
-
Short nameCD11, CD18 (FITC)
-
TechniqueFITC, Fluorescein
-
LabelFITC
-
Alternative nameAnti-CD11, CD18 (FITC)
-
Alternative techniquefluorescine
-
Gene info
-
Identity
-
Gene
-
Long gene nameintegrin subunit beta 2
-
Synonyms gene
-
Synonyms gene name
- integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
- integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
RefSeq identity
-
Classification
- Integrin beta subunits
- Complement system regulators and receptors
- CD molecules
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
-
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data