SI-CLP, Rat Mouse Monoclonal antibody-Human; Clone: 1C11, flow, Alexa Fluor 647 conj.
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Catalog numberYSRTMCA2619A647
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PricePlease ask
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Size1000ul
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Alternative name 1SI-CLP, Rat Mab anti-Human; Clone: 1C11, flow, Alexa Fluor 647 conj.
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Alternative name 2SI-CLP, Rat Mab antibody-Human; Clone: 1C11, flow, Alexa Fluor 647 conj.
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Alternative name 3SI-CLP, Rattus norvegicus Mab antibody-Homo sapiens; Clone: 1C11, flow, Alexa Fluor 647 conj.
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Alternative name 4anti-SI-CLP Clone: 1C11 flow Alexa Fluor 647
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Alternative name 5SI-CLP Clone: 1C11 flow Alexa Fluor 647
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate. Alexa Fluor 633 is a practical alternative to APC as well as Cy5. accurate-monoclonals made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the SI-CLP, Mouse Monoclonal antibody- ; Clone: 1C11, , conj. exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy. If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAlexa Fluor, ALEXA FLUOR 647
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolCHID1, SI, COTL1, CALML3, SLC2A4RG, CSRP3, PDLIM1, HEXIM1, PMEL
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Short nameSI-CLP, Mouse Monoclonal antibody- ; Clone: 1C11, , conj.
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TechniqueMouse monoclonal, Antibody, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostRat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelALEXA FLUOR 647
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SpeciesHuman, Humans, Mouses, Rats
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Alternative nameSI-CLP, Rat Mouse monoclonal (antibody to-)-H. sapiens; clonality: 1C11, flow, Alexa Fluor 647 conj.
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene namechitinase domain containing 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-10-27
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Entrez gene record
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RefSeq identity
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Classification
- Chitinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesucrase-isomaltase
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Synonyms gene name
- sucrase-isomaltase (alpha-glucosidase)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Glycoside hydrolase family 31
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecoactosin like F-actin binding protein 1
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Synonyms gene name
- coactosin-like 1 (Dictyostelium)
- coactosin-like F-actin binding protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-07-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecalmodulin like 3
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Synonyms gene name
- calmodulin-like 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-07-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSLC2A4 regulator
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecysteine and glycine rich protein 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-07-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namePDZ and LIM domain 1
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Synonyms gene
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Synonyms gene name
- PDZ and LIM domain 1 (elfin)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-01-21
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Entrez gene record
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Pubmed identfication
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Classification
- LIM domain containing
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHEXIM P-TEFb complex subunit 1
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Synonyms gene name
- hexamethylene bisacetamide inducible 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-06-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- P-TEFb complex subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepremelanosome protein
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Synonyms gene
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Synonyms gene name
- silver (mouse homolog) like
- silver homolog (mouse)
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-06-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data