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Category
Primary Antibodies
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Long description
Two types of T cell antigen receptors (TCRs) are now known, the α/β, and the γ/δ heterodimer, which are expressed at the cell surface in association with the CD3 molecular complex. TCR-γ can occur in either disulphide-linked or non-disulphide-linked forms. The γ/δ T-cell receptor is present on a minor subset of T lymphocytes in peripheral blood, thymus, spleen, and lymph node. In man and Mouse, only a small part of the T lymphocytes of the peripheral blood (
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Antibody come from
11F2 (anti-TCR gamma delta-l) is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Balb/c mice immunized with protein A-Sepharose/anti-CD3/antigen complex.
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Other description
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Clone
11F2
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Antigen antibody binding interaction
Mouse anti T-Cell Receptor gamma delta Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Clone 11F2 recognizes all molecular forms of the gamma/delta T-cell receptor (TCR) desribed so far
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Research interest
Cell adhesion,Immunology
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Application
ELISA,Flow Cytometry,Immunohistochemistry (frozen),Immunoprecipitation
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Antibody s suited for
11F2 is useful for Enzyme Linked Immuno Sorbent Assays, Flow Cytometry, Immunohistochemistry on frozen sections and Immunoprecipitation. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Borst, J., Van Dongen, J., Bolhuis, R., Peters, P., Hafler, D., De Vries, E., Van de Griend, R. (1988). Distinct molecular forms of Human T cell receptor g/d detected on viable T cells by a monoclonal antibody. I. Exp. Med 167 1626-1644 Vroom, T., Scholte, G., Ossendorp, F., Borst, J. (1991). Tissue distribution of Human g/d T cells: No evidence for general epithelial tropism. J. Clon. Pathol. 44 1012-1017 Bos, J.D., Teunissen, M.B.M., Cairo, I., Krieg, S.R., Kapsenberg, M.L., Das, P.K., and Borst, J. (1990). T-cell receptor gamma delta bearing cells in normal Human skin J. Invest. Dermatol. 94: 37-42. Lanier, L., Ruitenberg, J., Bolhuis, R., Borst, J., Phillips, J., Testi, R. (1988). Structural and serological heterogeneity of g/d T cell antigen receptor expression in thymus and peripheral blood. Eur J Immunol. 18 1985-1992 Lanier, L, Serafini, A., Ruitenberg, J., et all. (1987) The gamma T-cell antigen receptor. J Clin Immunol. 7 429 Testi, R., Lanier, L. (1989). Functional expression of CD28 on T cell antigen receptor g/d-bearing T lymphocytes. Eur J Immunol 19 185 Lanier, L., Federspiel, N., Ruitenberg, J., Phillips, J., Allison, J., Weiss, A. (1987(. The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes: A CD3-associated disulfide-linked g chain heterodimer. J Exp Med 165 1076-1094. Voogt, P., Falkenburg, J., Fibbe, W., et al. (1989). Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor-/CD3-, T cell receptor gd+/CD3+ and T cell receptor-α/β+/CD3+ lymphocytes 142 1774-1780.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. For cells, cell lines and tissues in culture till half confluency. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
