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Long name
Rabbit Antibody against Zebrafish shha, Biotin
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Synonym name
shha Zebrafish Antibody
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Species reactivity
Zebrafish
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Tested applications recommended dilutions
ELISA
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Conjugation
Biotin
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Antibody isotype
IgG
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UniProt ID
Q92008
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant Zebrafish Sonic hedgehog protein A protein (24-197AA)
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Immunogen species
Zebrafish
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Alias
Sonic hedgehog protein A,SHHA,VHH-1,Sonic hedgehog protein A N-product,Sonic hedgehog protein A C-product,shha,shh,vhh1
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Background
Intercellular signal essential for a variety of patterning events during development. Signal produced by the notochord that induces somite patterning, dorso-ventral patterning of the brain and early patterning of the developing eyes. Displays floor plate-inducing activity. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO (By similarity).
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Clonality
Polyclonal antibody
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Shipping and storage
shha Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Sonic hedgehog protein A
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
