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Synonyms
FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
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Alternative_names
FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
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Description
Mediates delivery of 5-methyltetrahydrofolate to the interior of cells
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Recombinant
Yes
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Source
HEK293 cells
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Endotoxin Level
< 1.0 EU per/ug
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Activity Specifications test method
Measured by its binding ability in a functional ELISA.
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Biological activity
Immobilized Folic acid-BSA conjugate at 5 ug/mL (100 uL/well) can bind Human FOLR1, Fc Tag with a linear range of 10-78 ng/mL
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Binding Capacity
Immobilized Folic acid-BSA conjugate at 5 ug/mL (100 uL/well) can bind Human FOLR1, Fc Tag with a linear range of 10-78 ng/mL
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Molecular Weight
51.3 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
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Reconstitution Instructions
Reconstitute in sterile deionized water to a concentration of 50 ug/ml.
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Background Information
Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
FOLR1
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Short name
CellExp™ FOLR1, Fc Tag, Recombinant
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Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
Fc Tag
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Species
Humans
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Alternative name
H. sapiens CellExp™ folate receptor 1 (adult), fragment c detection labelled, H. sapiens Rec.
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Alternative technique
rec
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Alternative to gene target
folate receptor 1 (adult), FBP and FOLR, FOLR1 and IDBG-63244 and ENSG00000110195 and 2348, folic acid transporter activity, Plasma membranes, Folr1 and IDBG-202269 and ENSMUSG00000001827 and 14275
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