Recombinant Human Flavokinase / RFK Protein (His tag)

• Catalog number
  PKSH030327-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  RIFK
• Activity
  NA
• Sequence
  Met1-His155
• Fusion tag
  N-His
• Accession
  Q969G6
• Expressed Host
  E. coli
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  ＞90 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  19.5 kDa
• AP Mol Mass
  20 kDa
• Formulation
  Supplied as sterile 20mM Tris, 10% glycerol, pH 8.0
• Background
  Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Flavokinase   RFK   Protein   His   tag  
• Gene symbol
  RFK
• Short name
  Recombinant Flavokinase / RFK Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Flavokinase / RFK Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:30324
• Gene
  RFK
• Long gene name
  riboflavin kinase
• Synonyms
  • FLJ11149
  • RIFK
• GenBank acession
  • AK002011
• Locus
  9q21.13
• Discovery year
  2004-05-12
• Entrez gene record
  55312
• Pubmed identfication
  • 14580199
• RefSeq identity
  • NM_018339
• VEGA ID
  OTTHUMG00000020040

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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