GlnA, Glutamine synthetase

  • Catalog number
    AS01 018
  • Price
    Please ask
  • Size
    50 µl (16 mg/ml)
  • Available ordering format
    Liquid in PBS pH 8.0, 0.02% sodium azide
  • Immunogen
    KLH-conjugated synthetic peptide derived from available bacterial GlnA sequences with perfect conservation in alpha, beta, gamma Proteobacteria, Enterobacteria, Thermotogales, Low GC Gram+, Cyanobacteria (except weak conservation with Trichodesmium thiebautii) including Synechocystis PCC 6803 Q59981
  • Raised in
    Hen
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Total IgY
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    AS01 018S | GlnA protein standard for a quantitative western blot or as a positive controlRecommended secondary antibodycollection of antibodies to nitrogen metabolismAlgal protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1:5000 with standard ECL (WB)
  • Molecular weight expected аpparent
    53 kDa
  • Verified reactivity
    Synechococcus sp. 7942, Synechocystis sp. 6803
  • Possible reactivity
    alpha, beta, gamma proteobacteria, enterobacteria, thermotogales, euryarchaeotes, crenarchaeotes, Arthropsira sp. PCC 8005
  • No reactivity
    diatoms, eukaryotic GlnA
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biol. 154:413-422.Burns et al. (2006). Inorganic carbon repletion constrains steady-state light acclimation in the cyanobacterium Synechococcus elongatus. J. Phycol. 42:610-621.
  • Scientific context
    Glutamine synthetase (EC=6.3.1.2) is the key enzyme in the incorporation of mineral nitrogen into glutamine. Activity of this enzyme is controlled by adenylarion under conditions of abundant glutamine.
  • Notes
    Peptide target used to elicit this antibody has a weak, sporadic conservation with Glutamine Synthetase to III, antibody not expected to detect this enzyme. Weak conservation with some Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GLNRS), but this antibody is not expected to detect this enzyme.
  • Protein number
    P77961 , Q59981
  • TAIR number
    Refer to NCBI
  • Gene target
  • Short name
    GlnA, Glutamine synthetase
  • Alternative name
    GlnA, Glutamine synthetase
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