This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Target of this antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-TANK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Alternative names of antibody target:
TANK (NT), TRAF family member-associated NF-kappaB activator, I-TRAF
Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Mouse small intestine tissue lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
This antibody reacts with:
Human, mouse, rat
Clonality of antibody:
Synthetic peptide surrounding amino acid 413 of human TANK
How to handle this antibody:
The antibody solution should be gently mixed before use.
Members of TRAF family associate with activated TNF-R and CD40 and have been implicated to induce nuclear translocation of NFkB. TANK can bind to all three TRAFs and synergizing with TRAF2 to activate the NFkB signal cascade.
Form of antibody:
Concentration of antibody:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.