Rabbit anti Human J chain of dimeric IgA, conjugated with FITC, Secondary Antibodies

• Catalog number
  RAHu-J-FITC
• Price
  Please ask
• Size
  1ml

• Host Source
  Rabbit
• Clone Name
  Polyclonal
• Product Category
  Immunology
• Species Reactivity
  Human
• Application
  ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
• Immunogen
  Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity; Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
• Purification Method
  NA
• Shipping Conditions
  NA
• Storage conditions
  Rabbit anti Human J chain of dimeric IgA, conjugated with FITC, Secondary Antibodies lized at +4ÁC--at least 10 years. Reconstituted at or below -20ÁC--3-5 years. Reconstituted at +4ÁC--7 days.
• Datasheet link
  NA
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Properties
  This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
• Conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by Exalpha. Rabbit antibodies are very stable and can be stored for several days at room temperature. Exalpha adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  chain   dimeric   conjugated   with   Secondary   Antibodies  
• Short name
  Rabbit anti J chain of dimeric IgA, conjugated with FITC, Secondary Antibodies
• Technique
  anti human, Rabbit, anti, FITC, IgA, antibody to, anti human antibodies, antibody Conjugates, Fluorescein, IgAs
• Host
  Rabbit, Rabbits
• Isotype
  NA, IgA
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  production species: rabbit antibody to H. sapiens J epitope on dimeric Immunoglobulin A, coupled including fluorecein, secondary detector Antibody Antibodies
• Alternative technique
  rabbit-anti, antibodies, fluorescine, immunoglobulin-a

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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