Recombinant Human CD3 e/CD3E (C-6His)
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Catalog number
C578-500
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Price
Please ask
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Size
500 ug
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Description
Recombinant Human CD3 epsilon is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a 6His tag at the C-terminus.
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Species reactivity
Human
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Origin
Human cells
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Peptide sequence
DGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDVDHHHHHH
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Estimated molecular weight
12,79 kDa
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Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
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Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
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Shipping condition
Ambient/Room Temperature
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Package form
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
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Storage conditions
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
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Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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UniProt number
P07766
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CD3E
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Short name
Recombinant CD3 e/CD3E (C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Human CD3 epsilon/CD3E(C-6His)
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Alternative technique
rec
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Alternative to gene target
CD3e molecule, epsilon (CD3-TCR complex), IMD18 and T3E and TCRE, CD3E and IDBG-73617 and ENSG00000198851 and 916, protein heterodimerization activity, Cell surfaces, Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501, CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054
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Gene info
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Identity
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Gene
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Long gene name
CD3e molecule
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Synonyms gene name
- CD3e antigen, epsilon polypeptide (TiT3 complex)
- CD3e molecule, epsilon (CD3-TCR complex)
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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