M13 + fd + F1 Filamentous Phages antibody

• Catalog number
  10R-M101a
• Price
  Please ask
• Size
  500 µg

• Category
  Primary Antibody
• Antibody Subtype
  Monoclonal Antibodies
• Area of research
  Miscellaneous
• Type of Immunogen
  M13/fd/F1 filamentous phages antibodies were raised in mouse using fd phages from E.Coli F+ strain ..
• Raised in
  Mouse
• Specificity
  M13 phage
• Cross Reactivity
  This antibody recognizes an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT
• Isotypes
  IgG2b
• Clone
  B62-FE2
• Method of Purification
  Protein A affinity chromatography
• Form Buffer
  Reconstitute in 0.1 ml distilled water. (final solution contains 10 mM Tris-HCl buffer, pH 8.0, 300 mM NaCl).
• Storage
  Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze-thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; Phage Display; IF; WB
• Usage Recommendations
  ELISA: 1:50,000, Phage Display detection limit=10^7 phage particles
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Gene
  M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
• French translation
  anticorps

• Gene target
  M13   Filamentous   Phages  
• Short name
  M13 + fd + F1 Filamentous Phages antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  Mouse monoclonal M13 + fd + F1 Filamentous Phages antibody
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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