TSLP, human recombinant

  • Catalog number
    4896-20
  • Price
    Please ask
  • Size
    20 ug
  • Synonyms
    Thymic Stromal Lymphopoietin
  • Alternative_names
    Thymic Stromal Lymphopoietin
  • Description
    Induces the release of T cell-attracting chemokines from monocytes
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥97%
  • Assay
    SDS-PAGE
  • Purity
    ≥97%
  • Biological activity
    The ED₅₀ determined by the ability to induce proliferation of human Interleukin-7 receptor α & human TSLP Receptor co-transfected with mouse BaF3 cells was found to be less than 0.3 ng/ml.
  • Molecular Weight
    15.0 kDa
  • Storage Temp
    -20°C
  • Shipping
    Blue ice
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Sterile filtered and lyophilized from a concentrated solution (1 mg/ml) containing 130 mM NaCl, and 20 mM sodium phosphate, pH 7.4.
  • Reconstitution Instructions
    Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers
  • Background Information
    TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor α chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c(+) dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor α chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c(+) dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-α whereas down-regulating Interleukin-10 and IFN-γ play a role in the initiation of allergic inflammation. Recombinant human TSLP produced in E. coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    TSLP  
  • Gene symbol
    TSLP
  • Short name
    TSLP, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    thymic stromal lymphopoietin, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    thymic stromal lymphopoietin, TSLP and IDBG-36507 and ENSG00000145777 and 85480, cytokine activity, Extracellular, Tslp and IDBG-134008 and ENSMUSG00000024379 and 53603
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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