Lefty Antibody, ALEXA FLUOR 350
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Catalog number
bs-11236R-A350
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Price
Please ask
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Size
100ul
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Long name
Lefty Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
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Also known as
Anti-Lefty PAb ALEXA FLUOR 350
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Category
Conjugated Primary Antibodies
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Conjugated with
ALEXA FLUOR® 350
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
Lefty
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Specificity
This is a highly specific antibody against Lefty.
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Modification
Unmodified
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Modification Site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Lefty
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Gene ID Number
10637
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Tested applications
IF(IHC-P)
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Recommended dilutions
IF(IHC-P)(1:50-200)
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Crossreactivity
Human, Mouse, Rat
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the antigen
During vertebrate embryogenesis, a left right axis is established. Secreted growth factors of the TGF beta family, including gene products derived from nodal, lefty 1 and lefty 2, play crucial roles in establishing left right asymmetries. TGF beta (Transforming growth factor beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF beta actions are directed by ligand induced activation of TGF beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF beta superfamily, inhibits TGF beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF beta receptor. Lefty also inhibits events downstream from R Smad phosphorylation. Lefty provides a repressed state of TGF beta responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (41 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
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Purification
Purified by Protein A.
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Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
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Excitation emission
343nm/442nm
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Synonyms
Protein lefty-1; AI450052; Ebaf; Left right determination factor 1; left right determination factor; Left right determination factor B; left-right determination factor; LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1; left-right determination, factor B; LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B; LEFTB; LEFTY 1; Lefty; lefty-1; LEFTY1; LEFTY1, MOUSE, HOMOLOG OF antibody; LEFTYB; Protein lefty-B; RGD1561867; Stimulated by retinoic acid gene 3 protein; STRA3; TGF beta 4; TGF-beta-4; Tgfb4; Transforming growth factor beta 4; Transforming growth factor beta-4.
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Properties
For facs or microscopy Alexa 1 conjugate. Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor, ALEXA FLUOR 350
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French translation
anticorps
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Gene target
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Gene symbol
LEFTY2
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Short name
Anti-Lefty
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 350
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Alternative name
Lefty Polyclonal Antibody, ALEXA FLUOR 350
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Alternative technique
antibodies
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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