Mouse anti Desmin

• Catalog number
  MUB0400S
• Price
  Please ask
• Size
  1 mL

• Category
  Primary Antibodies
• Long description
  Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
• Antibody come from
  RD301 is a Mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal extract of Chicken gizzard.
• Other description
  Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
• Clone
  RD301
• Antigen antibody binding interaction
  Mouse anti Desmin Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Canine,Chicken,Hamster,Human,Mouse,Rabbit,Rat,Swine,Zebrafish
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
• Research interest
  Cancer,Cytoskeleton,Stem cell research
• Application
  Immunohistochemistry (frozen),Western blotting, immunocytochemistry
• Antibody s suited for
  RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:5 – 1:20 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Quax, W., van den Broek, L., Egberts, W. V., Ramaekers, F., and Bloemendal, H. (1985). Characterization of the Hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer, Cell 43, 327-38. Verhagen, A. P., Aalders, T. W., Ramaekers, F. C., Debruyne, F. M., and Schalken, J. A. (1988). Differential expression of Keratins in the basal and luminal compartments of Rat prostatic epithelium during degeneRation and regeneRation, Prostate 13, 25-38. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. Schaart, G., Viebahn, C., Langmann, W., and Ramaekers, F. (1989). Desmin and titin expression in early postimplantation Mouse embryos, Development 107, 585-96. 5. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. Schaart, G., Pieper, F. R., Kuijpers, H. J., Bloemendal, H., and Ramaekers, F. C. (1991). Baby Hamster kidney (BHK-21/C13) cells can express striated muscle type proteins, Differentiation 46, 105-15. Dispersyn, G. D., Geuens, E., Ver Donck, L., Ramaekers, F. C., and Borgers, M. (2001). Adult Rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts, Cardiovasc Res 51, 230-40. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22, 639-50.
• Protein number
  P17661
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Gene
  Desmins or DES proteins detect by anti-desmin antibodies and DES ELISA kits. DSM1 and DSM2 play an important role in Desminopathy. Desmin regulated Myofibrilate Myopathy is a protein filament mutation of muscle proteins.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Desmin  
• Gene symbol
  DES
• Short name
  Mouse anti Desmin
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG2b
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Desmin
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:2770
• Gene
  DES
• Long gene name
  desmin
• Synonyms
  • CMD1I
  • CSM1
  • CSM2
• Synonyms name
  • intermediate filament protein
• GenBank acession
  • AF521879
• Locus
  2q35
• Discovery year
  2001-06-22
• Entrez gene record
  1674
• Pubmed identfication
  • 2673923
  • 9736733
• RefSeq identity
  • NM_001927
• Classification
  • Intermediate filaments Type III
• VEGA ID
  OTTHUMG00000058924
• Locus Specific Databases
  • Leiden Muscular Dystrophy pages
  • LRG_380

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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