Refer to PubMed
Net weight (g)
Recommended buffer for storage
PBS, 50% glycerol, 0.09% sodium azide
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite. | Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (SPC-718) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa. Rabbit Anti-FNIP1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on C2C12 Cells (Mouse Myoblast cell line) (SPC-718) | Rabbit Anti-FNIP1 Antibody used in Western blot (WB) on Kidney (SPC-718)
Synthetic peptide from the mid-protein of Human FNIP1
Antibody's other name
FNIP1 Antibody, Folliculin-interacting protein 1 Antibody, Folliculin interacting protein 1 Antibody, KIAA1961 Antibody
Antibody's recommended dilutions for use
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
To be tested
Primary research fields
Cancer, Cell Cycle, mTOR Pathway, Cancer, Autophagy
Contact our support service to receive datasheet or other technical documentation.
Total weight (kg)
See included datasheet or contact our support service.
Certificate of analysis
A 1:1000 dilution of SPC-718 was sufficient for detection of FNIP1 in 15 µg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Strong expression is found in the heart, liver placenta, muscle, nasal mucosa, salivary gland and uvula and moderate expression in kidney and lung. Higher levels detected in clear cell renal cell carcinoma (RCC) and chromophobe RCC than in normal kidney tissue.
Antibody's full description
Rabbit Anti-Human FNIP1 Polyclonal
Country of production
Antibody's purified from
Peptide Affinity Purified
To be tested
Antibody in cell
Refer to PubMed
Detects ~130 kDa.
Blue Ice or 4°C