Description Amplite Colorimetric Biotin Quantitation Kit is used for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.
Shipping Room Temperature
Shelflife 6 months
Kit Components Component A: avidin ; Component B: HABA Assay Buffer; Component C: D-Biotin
Ex (nm) 500 nm
Em (nm) NA
Personal Protection Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Uses Laboratory chemicals *For Research Use Only*
Ordering To order Amplite Colorimetric Biotin Quantitation Kit, please use the Cat. Nr.5522 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Tips Our specialists recommend you to follow carefully the pre-registered instructions for Amplite Colorimetric Biotin Quantitation Kit
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Colorimetric assays or detection use UV absorption or enzymatic color reaction.
Short name Amplite Colorimetric Quantitation Kit
Alternative name Amplite colorimetry biotinilated Quantitation reagent
Alternative technique assays
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832