This antibody has been succesfully tested for use in following methods: Western blot, Immunoprecipitation, Immunohistochemistry
Target of this antibody: Phospho-ATF-2 (Thr71)
Formulation of this antibody: 100 µg (0.5 mg/ml) purified rabbit anti-Phospho-ATF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Alternative names of antibody target: ATF2, HB16, CREB2, CREBP1, CRE-BP1, CRE-BP1, TREB7, MGC111558, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; cAMP response element-binding protein CRE-BP1; HB16; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; CREB-2.
Recommended usage: Western blotting (1-4 µg/ml), immunoprecipitation (10 µg/ml), and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human, mouse, and rat origins.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse, rat
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps