Mouse Anti-Mouse Notch1 Monoclonal IgG1, Clone S253-32
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Catalog numberSMC-430D-APC
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PricePlease ask
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Size100 µg
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CloneS253-32
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ImmunogenFusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
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Antibody s full descriptionMouse Anti-Mouse Notch1 Monoclonal IgG1 Antibody, Clone: S253-32: APC
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameNeurogenic locus notch homolog protein 1 Antibody, hN1 Antibody, Translocation associated notch protein Antibody, TAN1 Antibody, TAN-1 Antibody, Motch A Antibody, mT14 Antibody, p300 Antibody, Motch Antibody, Mis6 Antibody, Neurogenic locus notch protein homolog Antibody, NICD Antibody, Notch 1 intracellular domain Antibody, Notch gene homolog 1 (Drosophila) Antibody, Notch homolog 1 Antibody, translocation-associated (Drosophila) Antibody, NOTCH Drosophila Antibody, homolog of 1 Antibody, Translocation associated notch homolog Antibody, Translocation associated notch protein TAN 1 Antibody, xotch Antibody
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Raised inMouse
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Antibody s targetNotch1
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Primary research fieldsNeuroscience, Cell Signaling, Epigenetics, cardiovascular System, Heart
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityMouse, Rat
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Antibody s dilutionsWB (1:1000); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects >270kDa, ~120kDa and small fragments due to proteolysis. Does not cross-react with Notch 2 or Notch3.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Nucleus
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextNotch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
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Bibliography1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719. 2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company. 3. Lindsell C.E., et al. (1995) Cell 80: 909-917. 4. Uyttendaele H., et al. (1996) Development 122: 2251-2259. 5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.
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Released date15-Feb-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_032740.3
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Gene number18128
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Protein numberQ01705
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32 (SMC-430). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody (SMC-430) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Notch1 Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32 (SMC-430). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 µg). . Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody (SMC-430) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa. Mouse Anti-Notch1 Antibody [S253-32] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-430) Mouse Anti-Notch1 Antibody [S253-32] used in Western Blot (WB) on Rat Brain Membrane (SMC-430)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGKV1D-32, SNORD115-32, PPP1R1B, IGHV3-32, IGKV1-32, IGLV3-32, ZNF138, CUTC, LINC01234, LUARIS
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Short nameMouse Anti-Mouse Notch1 Monoclonal IgG1, Clone S253-32
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelAPC
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse notch 1 monoclonal IgG1, clonality S253-32
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Alternative techniquemurine, antibodies
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Alternative to gene targetnotch 1, AOS5 and AOVD1 and hN1 and TAN1, NOTCH1 and IDBG-92445 and ENSG00000148400 and 4851, sequence-specific DNA binding, nuclei, Notch1 and IDBG-149571 and ENSMUSG00000026923 and 18128, NOTCH1 and IDBG-640538 and ENSBTAG00000022799 and 767866
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Clone nameS253-32
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1D-32
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-32
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory inhibitor subunit 1B
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 1B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-32 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-32
- immunoglobulin heavy variable 3-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1-32 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 1-32
- immunoglobulin kappa variable 1-32 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 3-32 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 3-32
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namezinc finger protein 138
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Synonyms gene name
- zinc finger protein 138 (clone pHZ-32)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-02-11
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Entrez gene record
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecutC copper transporter
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Synonyms gene name
- cutC copper transporter homolog (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-04-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1234
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Synonyms
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Locus
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Discovery year2014-02-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelncRNA upregulator of antiviral response interferon signaling
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Synonyms
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Synonyms name
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Locus
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Discovery year2017-03-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
