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Also known as
CD45RO
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Other names
Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase isozyme L1; OTTHUMP00000125219; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
UCHL1; UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5; N/A
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Gene name synonims
UCHL1; UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5; N/A
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Other gene names
UCHL1; UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
UCHL-1
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
The UCHL1 antibody can be used in lymphoma diagnosis and is clustered as CD45RO, present on most thymocytes, a subset of T cells (CD4 and CD8), monocytes and granulocytes. The antigen is not expressed on B cells. The antibody can be used on frozen and paraffin sections, and cell prepaRations using flow cytometry. The advised dilution is 1:5 to 1:10 with no pretreatment (based on the indirect method), but with an ABC method the dilution can be 5-10 times higher. The optimal dilution should, however, be tested by serial dilution. Dilute immediately before use with PBS or Tris HCl. pH 7.4. Immunoblotting: the antibody is reactive with a 185 kD protein.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Each vial contains 1 ml culture supernatant containing 0.09% sodium azide, provided azide-free upon request.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius., or in small aliquots the antibody should be stored at -20 degrees Celsius..
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Tested applications
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
