Target of this antibody:
Alternative names of antibody target:
Proteinase K is a serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process.
Clonality of antibody:
This antibody reacts with:
Recombinant proteinase K
Formulation of this antibody:
30 µg (0.5 mg/ml) polyclonal antibody in PBS pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Affinity purified antibody
This antibody has been succesfully tested for use in following methods:
Western blotting (3-6 µg/ml)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).