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Rabbit Anti Human Smad2 Polyclonal

Rabbit Anti Human Smad2 Polyclonal is available 15 times from Stressmark antibodies labs

SPC-1284D-ALP | Rabbit Anti-Human Smad2 Polyclonal: Alkaline Phosphatase size: 100 µl | 397.99 USD

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SPC-1284D-APC | Rabbit Anti-Human Smad2 Polyclonal: APC size: 100 µl | 404.81 USD

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SPC-1284D-A390 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 390 size: 100 µl | 407.08 USD

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SPC-1284D-A488 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 488 size: 100 µl | 405.94 USD

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SPC-1284D-A565 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 565 size: 100 µl | 405.94 USD

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SPC-1284D-A594 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 594 size: 100 µl | 405.94 USD

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SPC-1284D-A633 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 633 size: 100 µl | 405.94 USD

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SPC-1284D-A655 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 655 size: 100 µl | 405.94 USD

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SPC-1284D-A680 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 680 size: 100 µl | 405.94 USD

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SPC-1284D-A700 | Rabbit Anti-Human Smad2 Polyclonal: ATTO 700 size: 100 µl | 405.94 USD

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SPC-1284D-BI | Rabbit Anti-Human Smad2 Polyclonal: Biotin size: 100 µl | 400.26 USD

Catalog number SPC-1284D-BI
Price 400.26 USD
Size 100 µl
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test StressMark antibodies supplies antibodies that are for research of human proteins.
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation Biotinylated
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetSmad2
Short name Rabbit Anti- Smad2 Polyclonal:
Technique anti-Human, Polyclonal, Rabbit, anti
Label Biotin
Alternative name production species: rabbit antibody to-H. sapiens SMAD family member 2 polyclonal: biotinilated
Alternative to gene target SMAD family member 2, hMAD-2 and hSMAD2 and JV18 and JV18-1 and MADH2 and MADR2, SMAD2 and IDBG-3127 and ENSG00000175387 and 4087, transforming growth factor beta receptor, nuclei, Smad2 and IDBG-159056 and ENSMUSG00000024563 and 17126, SMAD2 and IDBG-644642 and ENSBTAG00000039916 and 515125,516010
MeSH Data
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Product images
    #Human#.jpg

SPC-1284D-FITC | Rabbit Anti-Human Smad2 Polyclonal: FITC size: 100 µl | 395.71 USD

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SPC-1284D-PCP | Rabbit Anti-Human Smad2 Polyclonal: PerCP size: 100 µl | 404.81 USD

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SPC-1284D-RPE | Rabbit Anti-Human Smad2 Polyclonal: RPE size: 100 µl | 401.40 USD

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SPC-1284D | Rabbit Anti-Human Smad2 Polyclonal: Unconjugated size: 100 µl | 351.36 USD

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PubMed number Refer to PubMed
Net weight (g) 0.1
Recommended buffer for storage PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Antibody's reactivity Human, Mouse, Rat
Warning information Non-hazardous
Representative figure legend Western blot analysis of Human Hek293 cell lysates showing detection of ~52kDa Smad2 protein using Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284) at 1:1000. Predicted/Observed Size: ~52kDa. Rabbit Anti-Smad2 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1284)
Raised in Rabbit
NCBI number NP_005892.1
Gene number 4087
Released date 6/Sep/2016
Immunogen A synthesized peptide derived from human Smad2
Antibody's other name hMAD-2 Antibody, JV18 Antibody, MAD Antibody, MAD homolog 2 Antibody, SMAD 2 Antibody, SMAD family member 2 Antibody
Protein number Q15796
Antibody's recommended dilutions for use WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Tested applications To be tested
Primary research fields Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic
Antibody's datasheet Contact our support service to receive datasheet or other technical documentation.
Total weight (kg) 1.4
Stock availabilit In Stock
Scientific context Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
Antibody's target Human Smad2
Certificate of analysis A 1:1000 dilution of SPC-1284 was sufficient for detection of Smad2 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Tissue specificity Expressed at high levels in skeletal muscle, heart and placenta.
Antibody's full description Rabbit Anti-Human Smad2 Polyclonal
Country of production Canada
Antibody's purified from Peptide Affinity Purified
Antibody's concentration See included datasheet or contact our support service
Tested reactivity To be tested
Antibody in cell Cytoplasm, Nucleus
Antibody's category Polyclonal Antibodies
Bibliography 1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.
Verified applications WB, IHC, ICC/IF
Antibody's specificity Detects ~58kDa, endogenous levels of total Smad2
Storage recommendations -20°C
Shipping recommendations Blue Ice or 4°C
Host Rabbit
Species Human
Alternative technique polyclonals
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