Refer to PubMed
Net weight (g)
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Human, Mouse, Rat
Representative figure legend
Western blot analysis of Human Hek293 cell lysates showing detection of ~52kDa Smad2 protein using Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284) at 1:1000. Predicted/Observed Size: ~52kDa. Rabbit Anti-Smad2 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1284)
A synthesized peptide derived from human Smad2
Antibody's other name
hMAD-2 Antibody, JV18 Antibody, MAD Antibody, MAD homolog 2 Antibody, SMAD 2 Antibody, SMAD family member 2 Antibody
Antibody's recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
To be tested
Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic
Contact our support service to receive datasheet or other technical documentation.
Total weight (kg)
Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
Certificate of analysis
A 1:1000 dilution of SPC-1284 was sufficient for detection of Smad2 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Expressed at high levels in skeletal muscle, heart and placenta.
Antibody's full description
Rabbit Anti-Human Smad2 Polyclonal
Country of production
Antibody's purified from
Peptide Affinity Purified
See included datasheet or contact our support service
To be tested
Antibody in cell
1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.
WB, IHC, ICC/IF
Detects ~58kDa, endogenous levels of total Smad2
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