Anti-DPYD (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-8288R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  DPYD
• Specificity
  This antibody reacts specifically with DPYD
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human DPYD
• Gene ID
  1806
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Dihydropyrimidine dehydrogenase (DPYD) catalyzes the first rate-limiting step of the NADPH-dependent catabolism of uracil and thymine to dihydrouracil and dihydrothymine; thus, a deficiency of DPYD leads to an accumulation of uracil and thymine. Abnormal concentrations of these metabolites in bodily fluids may be the cause of neurological disease and a contraindication for treatment of cancer patients with certain pyrimidine analogs. DPYD also catalyzes the anticancer agent 5-fluorouracil (5-FU) pathway and is involved in the efficacy and toxicity of 5-FU. Variations in DPYD concentration may arise from alterations at the transcriptional level of the dihydropyrimidine dehydrogenase gene. Specifically, hypermethylation of the DPYD promoter downregulates dihydropyrimidine dehydrogenase expression. Deficient DPYD alleles may constitute a risk factor for severe toxicity following treatment with 5-FU.Involvement in disease:Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) ; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  DHP; DHPDHase; Dihydropyrimidine dehydrogenase [NADP+]; Dihydropyrimidine dehydrogenase; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase; DPD; DPYD; DPYD_HUMAN; MGC132008; MGC70799; OTTHUMP00000058954.
• Also known as
  DPYD Polyclonal Antibody
• Other name
  Anti-DPYD Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  DPYD  
• Gene symbol
  DPYD-AS2, DPYD-AS1, DPYD-IT1, DPYD
• Short name
  Anti-DPYD
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  DPYD Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:40196
• Gene
  DPYD-AS2
• Long gene name
  DPYD antisense RNA 2
• Synonyms gene name
  • DPYD antisense RNA 2 (non-protein coding)
• Locus
  1p21.3
• Discovery year
  2011-05-20
• Entrez gene record
  100873933
• Pubmed identfication
  • 28391028
• RefSeq identity
  • NR_046591
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000039680

Gene info

• Identity
  HGNC:40195
• Gene
  DPYD-AS1
• Long gene name
  DPYD antisense RNA 1
• Synonyms gene name
  • DPYD antisense RNA 1 (non-protein coding)
• Locus
  1p21.3
• Discovery year
  2011-05-20
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000039682

Gene info

• Identity
  HGNC:41326
• Gene
  DPYD-IT1
• Long gene name
  DPYD intronic transcript 1
• Synonyms gene name
  • DPYD intronic transcript 1 (non-protein coding)
• Locus
  1p21.3
• Discovery year
  2011-05-20
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000039679

Gene info

• Identity
  HGNC:3012
• Gene
  DPYD
• Long gene name
  dihydropyrimidine dehydrogenase
• Synonyms
  • DPD
  • DHPDHase
• Synonyms name
  • Dihydrothymine dehydrogenase
  • Dihydrouracil dehydrogenase
• GenBank acession
  • U20938
• Locus
  1p21.3
• Discovery year
  1994-07-07
• Entrez gene record
  1806
• Pubmed identfication
  • 7713523
• RefSeq identity
  • NM_000110
• VEGA ID
  OTTHUMG00000039683
• Locus Specific Databases
  • UMD Locus Specific Databases
  • LRG_722

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Product files

Similar products